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KMID : 0861020200350040009
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Korea Journal of Herbology
2020 Volume.35 No. 4 p.9 ~ p.16
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A Study on the Effect of Liriopis tuber water extracton Hydrogen Peroxide-stimulated C6 Astrocyte Cells
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Park Ki-Ho
Kang Seok-Yong
Jung Hyo-Won
Park Yong-Ki
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Abstract
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Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H2O2-induced oxidative damage in C6 rat astrocytes.
Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 ㎎/㎖ or without for 30 min and then stimulated with H2O2 at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope.
Results : LT extract induced cell proliferation in H2O2-stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H2O2-stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H2O2-stimulated C6 cells.
Conclusions : LT extract can regulate H2O2-induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.
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KEYWORD
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Liriopis tuber, Liriope platyphylla, C6 astrocytes, oxidative damage, STAT3, Akt, HO-1, PKCα
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